Difference between revisions of "Bio Lab Infrastructure"
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− | == How to start a art-sci bio lab? == | + | [[File:art_sci_lab.jpg|320px|right]] |
+ | == How to start a DIWO art-sci bio lab? == | ||
+ | '''If you can't build your lab, you don't own the lab''' | ||
− | [[ | + | See a version from [[Dusjagr|Marc Dusseiller aka dusjagr]], Workshopologist and from [[Gaudi|Urs Gaudenz]], Dipl. Ing. Mikrotechnik |
+ | {{#widget:Iframe | ||
+ | |url=https://www.slideshare.net/slideshow/embed_code/key/BI5g7PR5Kfw1lk | ||
+ | |width=400 | ||
+ | |height=320 | ||
+ | |border=0 | ||
+ | }} | ||
+ | |||
+ | {{#widget:Iframe | ||
+ | |url=https://www.slideshare.net/slideshow/embed_code/key/d1PH1OqSge3SM5 | ||
+ | |width=400 | ||
+ | |height=320 | ||
+ | |border=0 | ||
+ | }} | ||
+ | |||
+ | See an old version of the [http://www.slideshare.net/slideshow/embed_code/31019355 slides] | ||
+ | |||
+ | === People not Infrastructure === | ||
+ | |||
+ | * '''Lab Making | The Book: HackteriaLab 2014 - Yogyakarta''', by Urs Gaudenz, Sachiko Hirosue | ||
+ | |||
+ | [http://wlu18www30.webland.ch/wiki/images/9/91/LabMaking_HLab14book.pdf Instructions on lab-making] | ||
+ | |||
+ | ''"Making a Laboratory is both a spontaneous activity and a slow labor of love."'' | ||
+ | |||
+ | A Lab is a place to labor (not only of love), experiment, research and share - there is no labor, experiment, research or sharing without the people. A Lab is not a museum for journalists, but a place made by a person for other people Start a small Lab , put it in a box, suitcase, backpack, picnic basket and take it with you. A Mobile Lab it actually allows you to go to people, and not wait for the others to come. | ||
+ | |||
+ | Now also on [https://medium.com/@Sachiko/lab-making-d573afa93231 Medium], revised edition. | ||
+ | |||
+ | [[File:lab-luggages.jpg|400px]] | ||
+ | |||
+ | * '''Lab Infrastructure / Equipment''' | ||
+ | |||
+ | Experiences from setting up the [[BioTehna Lab Infrastructure]]. People not equipment. 20% Science 80% Rock'n'Roll. | ||
+ | |||
+ | Materials list for electronic prototyping from ali-express: [https://docs.google.com/spreadsheets/d/1vUl_qyV9iyexbR1D3tLbsDPX_wXkZAN80uj0P9YWlUo/edit here] | ||
+ | |||
+ | === Thoughts about Infrastructural Hospitality === | ||
+ | |||
+ | People putting boxes to make people feel comfortable... like someone running a restaurant, make sure your people are well fed and feel comfortable. | ||
+ | |||
+ | === Biosafety and other good practices === | ||
+ | |||
+ | We have collected a lot of information about [[DIY-BSL1]] and specificially looked at the regulations in Switzerland and how to use the [[DIY-BSL1 Manual Ecogen]] to register you lab for level 1 experiments using genetically modified microbes. Also specifically for [[RandeLab-GMP]] we have played through some of the concepts of ''"Gemütliche Mikrobiologische Praxis"''. | ||
==Infrastructure == | ==Infrastructure == | ||
+ | |||
+ | ===BioLab Equipment=== | ||
+ | |||
+ | <div class="alertBox1"> | ||
+ | |||
+ | {| class="alert" | ||
+ | |||
+ | !image | ||
+ | !# | ||
+ | !what | ||
+ | !notes | ||
+ | |||
+ | |- | ||
+ | |[[File:BT_hotplate.jpg|120px]] ||1 ||small hotplate|| | ||
+ | |||
+ | |- | ||
+ | |[[File:BT_mixer.jpg|120px]] ||1 ||Mixer || | ||
+ | |||
+ | |- | ||
+ | |[[File:BT_balance.jpg|120px]] ||1 ||small balance 0.02g - 1000g || | ||
+ | |||
+ | |- | ||
+ | |[[File:BT_hotdog_machine.jpg|120px]] ||1 ||HotDog machine ||it's like a small cooker/pot | ||
+ | |||
+ | |- | ||
+ | |[[File:BT_grinder.jpg|120px]] ||1 ||Coffee grinder || thanks bordul :-) | ||
+ | |||
+ | |- | ||
+ | |[[File:BT_DIY_micrscope.jpg|120px]] ||1 ||DIY microscope PS3eye || made by tamberg, used by many | ||
+ | |||
+ | |- | ||
+ | |[[File:BT_DIY_micrscopy-kit.jpg|120px]] ||2 ||DIY Microscopy kits || made by gaudi, [[Laser-cut microscopy stages]] | ||
+ | |||
+ | |- | ||
+ | |[[File:BT_DIY_spectroscope.jpg|120px]] ||1 ||DIY Spectroscope PS3eye || made by Bengt, check [http://publiclaboratory.org/wiki/video-spectrometer public laboratory] | ||
+ | |||
+ | |- | ||
+ | |[[File:BT_mycelium_scanner.jpg|120px]] ||1 ||scanner with embeddedincubator || made by bostjan et al at [http://hackteria.org/?p=1675 NanoSmano 3] | ||
+ | |||
+ | |} | ||
+ | |||
+ | </div> | ||
+ | |||
+ | ===BioLab stuff=== | ||
+ | |||
+ | <div class="alertBox1"> | ||
+ | |||
+ | {| class="alert" | ||
+ | |||
+ | !image | ||
+ | !# | ||
+ | !what | ||
+ | !notes | ||
+ | |||
+ | |- | ||
+ | |[[File:BT_glassware.jpg|120px]] ||4 x Erlenmayer ||0 x beakers || 1 x 100ml graded cylinder | ||
+ | |||
+ | |- | ||
+ | |[[File:BT_micropipette.jpg|120px]] ||1 ||fucked up 100µl micropipette |check online free samples or chinese stuff | ||
+ | |||
+ | |- | ||
+ | |[[File:BT_pots.jpg|120px]] ||2 ||small cooking pots ||we need a steampot for sterilization! | ||
+ | |||
+ | |- | ||
+ | |[[File:BT_plasticware.jpg|120px]] ||var. ||kitchen plastic ware ||many tupperware | ||
+ | |||
+ | |- | ||
+ | |[[File:BT_DIY_UV-drawer.jpg|120px]] ||1 x UV light bulp (black light) ||1 x 254nm / 366nm tube || 4 x UV-LED's | ||
+ | |||
+ | |- | ||
+ | |[[File:BT_DIY_gel_electro.jpg|120px]] ||1 ||DIY gel-box || var. Food colours || 1 pack of Agar Agar || we need more agar! | ||
+ | |||
+ | |} | ||
+ | |||
+ | </div> | ||
===General Technical Equipment=== | ===General Technical Equipment=== | ||
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|- | |- | ||
|[[File:BT_dremel.jpg|120px]] ||1 ||Cordless Dremel and toolsl ||belongs to dusjagr | |[[File:BT_dremel.jpg|120px]] ||1 ||Cordless Dremel and toolsl ||belongs to dusjagr | ||
+ | |||
+ | |- | ||
+ | |[[File:BT_labelling.jpg|120px]] ||1 ||Dymo LetraTag ||we need green ammo, from kniigica next to nama | ||
|} | |} | ||
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!image | !image | ||
− | ! | + | !Stuff |
− | ! | + | !thing 1 |
− | ! | + | !thing 2 |
+ | !thing 3 | ||
+ | !thing 4 | ||
+ | !thing 5 | ||
|- | |- | ||
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|- | |- | ||
− | |[[File:BT_Eksperiment.jpg|120px]] || | + | |[[File:BT_Eksperiment.jpg|120px]] ||5 x breadboards ||2 x multimeters ||crocodile cables || Jumper wires |
|- | |- | ||
− | |[[File:BT_glues.jpg|120px]] || | + | |[[File:BT_glues.jpg|120px]] ||2 x large glue gun (+ammo)||1 x small glue gun (+ammo)||1 x epoxy || rubber bands || cable binders || get more small glue guns and loads of ammo |
|- | |- | ||
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|- | |- | ||
|[[File:BT_helping_hands.jpg|120px]] ||5 x Helping hands ||2 x Unsoldering tools ||what else? | |[[File:BT_helping_hands.jpg|120px]] ||5 x Helping hands ||2 x Unsoldering tools ||what else? | ||
+ | |||
+ | |} | ||
+ | |||
+ | </div> | ||
+ | |||
+ | ===Electronics and Parts=== | ||
+ | |||
+ | [[File:BT_electronics.jpg|200px|right|thumb|RAKO boxes labelled with Hackteria / Kapelica]] | ||
+ | |||
+ | <div class="alertBox1"> | ||
+ | |||
+ | {| class="alert" | ||
+ | |||
+ | !image | ||
+ | !Class | ||
+ | !Group 1 | ||
+ | !Group 2 | ||
+ | !Group 3 | ||
+ | !Group 4 | ||
+ | !Group 5 | ||
+ | |||
+ | |- | ||
+ | |[[File:BT_Electronics1_parts.jpg|120px]] || Chips || 10 attiny 85 || 20 4093 || various | ||
+ | |||
+ | |- | ||
+ | |[[File:BT_uinos.jpg|120px]] ||4 x arduinos ||Table lamps ||get 10 large USB cables, and more arduinos | ||
+ | |||
+ | |- | ||
+ | |[[File:BT_chips.jpg|120px]] ||Chips || 10 attiny 85 || 20 4093 || various | ||
+ | |||
+ | |- | ||
+ | |[[File:BT_mignon_gamekit.jpg|120px]] ||2x ||Mignon Gamekit ||3 x Programmers || see info on [http://www.mignongamekit.com/] | ||
|} | |} | ||
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|- | |- | ||
− | |[[File:Box_BioCyberKidzz.jpg|120px]] ||1 ||loads of fun stuff ||see [ | + | |[[File:Box_BioCyberKidzz.jpg|120px]] ||1 ||loads of fun stuff ||see [http://hackteria.org/wiki/index.php/Hackteria_%26_SGMK_BioCyberKidzz#BioLab_Ingredients_List BioCyberKidzz] |
|} | |} | ||
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</div> | </div> | ||
− | == Where to get stuff == | + | == Wishlist == |
+ | |||
+ | ===Lab Gear=== | ||
+ | |||
+ | - trash bin for separating garbage | ||
+ | - incubator | ||
+ | - fridge with freezer | ||
+ | - additional shelves | ||
+ | - DIY PCR | ||
+ | - couple of shelves | ||
+ | - centrifuge | ||
+ | - OD meter | ||
+ | |||
+ | ===Stuff=== | ||
+ | |||
+ | |||
+ | == Where to get stuff (in Ljubljana) == | ||
===Organisms=== | ===Organisms=== | ||
Line 122: | Line 296: | ||
http://www.sanolabor.si/ | http://www.sanolabor.si/ | ||
+ | |||
+ | http://market.mikro-polo.si/ | ||
+ | |||
+ | http://dx.com/p/mg16129-c-5-led-auxiliary-clip-magnifier-black-white-eu-plug-110-240v-3-x-aa-193552 | ||
===Tools=== | ===Tools=== | ||
Line 132: | Line 310: | ||
http://www.conrad.si/ (supposedly too expensive) | http://www.conrad.si/ (supposedly too expensive) | ||
+ | |||
+ | http://dx.com/ order gadgets and electronics from china/HK | ||
===A/V equipment=== | ===A/V equipment=== | ||
Line 142: | Line 322: | ||
http://www.arhiv.mop.gov.si/si/o_ministrstvu/organiziranost/direktorat_za_okolje/ | http://www.arhiv.mop.gov.si/si/o_ministrstvu/organiziranost/direktorat_za_okolje/ | ||
+ | |||
+ | == Other links on how to start a DIWO BioHackArt lab == | ||
+ | |||
+ | From the [http://waag.org/en/event/do-it-together-bio-build-open-wetlab-2 Waag Do-It-Together biolab] check their [[File:waag_wishlist.pdf]] | ||
+ | |||
+ | [http://scienceblogs.com/worldsfair/2009/04/09/using-ebay-to-set-up-a-molecul/ Using eBay to set up a molecular biology lab: costs less than $1000!] | ||
+ | |||
+ | [http://openwetware.org/wiki/DIYbio/FAQ/Equipment exerpts from the DIYbio maillist assembled on openWetWare] | ||
+ | |||
+ | [http://www.instructables.com/id/Set-Up-Your-Home-Chemistry-Lab/?ALLSTEPS Instructable how to set up a home chemistry lab] | ||
+ | |||
+ | [http://blog.makezine.com/setting-up-a-home-science-laborator/ makezine, setting up a home science lab part 1] [http://blog.makezine.com/setting-up-a-home-science-lab2/ part 2], [http://blog.makezine.com/setting-up-a-home-science-lab2/ part 3] | ||
+ | |||
+ | [http://www.indiebiotech.com/?page_id=53 From IndieBiotech aka Cathal Garvey] not much on it, but a start. | ||
+ | |||
+ | more of [https://github.com/cathalgarvey/ Cathal's protocolls on github] | ||
+ | |||
+ | === Good Microbiological Practice === | ||
+ | |||
+ | http://www.lbl.gov/ehs/biosafety/manual/html/AppxD.shtml | ||
+ | |||
+ | === Next level shit... how to get BSL1 === |
Latest revision as of 03:57, 25 April 2020
Contents
How to start a DIWO art-sci bio lab?
If you can't build your lab, you don't own the lab
See a version from Marc Dusseiller aka dusjagr, Workshopologist and from Urs Gaudenz, Dipl. Ing. Mikrotechnik
See an old version of the slides
People not Infrastructure
- Lab Making | The Book: HackteriaLab 2014 - Yogyakarta, by Urs Gaudenz, Sachiko Hirosue
"Making a Laboratory is both a spontaneous activity and a slow labor of love."
A Lab is a place to labor (not only of love), experiment, research and share - there is no labor, experiment, research or sharing without the people. A Lab is not a museum for journalists, but a place made by a person for other people Start a small Lab , put it in a box, suitcase, backpack, picnic basket and take it with you. A Mobile Lab it actually allows you to go to people, and not wait for the others to come.
Now also on Medium, revised edition.
- Lab Infrastructure / Equipment
Experiences from setting up the BioTehna Lab Infrastructure. People not equipment. 20% Science 80% Rock'n'Roll.
Materials list for electronic prototyping from ali-express: here
Thoughts about Infrastructural Hospitality
People putting boxes to make people feel comfortable... like someone running a restaurant, make sure your people are well fed and feel comfortable.
Biosafety and other good practices
We have collected a lot of information about DIY-BSL1 and specificially looked at the regulations in Switzerland and how to use the DIY-BSL1 Manual Ecogen to register you lab for level 1 experiments using genetically modified microbes. Also specifically for RandeLab-GMP we have played through some of the concepts of "Gemütliche Mikrobiologische Praxis".
Infrastructure
BioLab Equipment
image | # | what | notes |
---|---|---|---|
1 | small hotplate | ||
1 | Mixer | ||
1 | small balance 0.02g - 1000g | ||
1 | HotDog machine | it's like a small cooker/pot | |
1 | Coffee grinder | thanks bordul :-) | |
1 | DIY microscope PS3eye | made by tamberg, used by many | |
2 | DIY Microscopy kits | made by gaudi, Laser-cut microscopy stages | |
1 | DIY Spectroscope PS3eye | made by Bengt, check public laboratory | |
1 | scanner with embeddedincubator | made by bostjan et al at NanoSmano 3 |
BioLab stuff
General Technical Equipment
Electronics and Parts
image | Class | Group 1 | Group 2 | Group 3 | Group 4 | Group 5 |
---|---|---|---|---|---|---|
Chips | 10 attiny 85 | 20 4093 | various | |||
4 x arduinos | Table lamps | get 10 large USB cables, and more arduinos | ||||
Chips | 10 attiny 85 | 20 4093 | various | |||
2x | Mignon Gamekit | 3 x Programmers | see info on [1] |
Boxes
image | content | content | content | Content | notes |
---|---|---|---|---|---|
5 x Soldering Irons | 1 x Solder wire | 5 x sponges | anything else? | ||
2 x Table lamps | 2 x Power Plugs | 3 x 220V Timers | 3 x remote control 220V Timers | ||
1 | loads of fun stuff | see BioCyberKidzz |
Wishlist
Lab Gear
- trash bin for separating garbage - incubator - fridge with freezer - additional shelves - DIY PCR - couple of shelves - centrifuge - OD meter
Stuff
Where to get stuff (in Ljubljana)
Organisms
http://lgcstandards-atcc.org/Default.aspx?base
Chemicals
Slovenian distributors of lab chemicals, including enzymes and stuff
Everything you ever wanted to know about agar http://www.fao.org/docrep/X5822E/x5822e03.htm
Labware
http://dx.com/p/mg16129-c-5-led-auxiliary-clip-magnifier-black-white-eu-plug-110-240v-3-x-aa-193552
Tools
Materials
Electronics
http://www.conrad.si/ (supposedly too expensive)
http://dx.com/ order gadgets and electronics from china/HK
A/V equipment
Helpful local institutions, companies and other people we have had pleasant interactions with in the past
http://ibk.mf.uni-lj.si/defaultslo.html
http://www.arhiv.mop.gov.si/si/o_ministrstvu/organiziranost/direktorat_za_okolje/
Other links on how to start a DIWO BioHackArt lab
From the Waag Do-It-Together biolab check their File:Waag wishlist.pdf
Using eBay to set up a molecular biology lab: costs less than $1000!
exerpts from the DIYbio maillist assembled on openWetWare
Instructable how to set up a home chemistry lab
makezine, setting up a home science lab part 1 part 2, part 3
From IndieBiotech aka Cathal Garvey not much on it, but a start.
more of Cathal's protocolls on github
Good Microbiological Practice
http://www.lbl.gov/ehs/biosafety/manual/html/AppxD.shtml