Difference between revisions of "Step 1 - Taking dry DNA from wells"

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(Created page with '1. Making sure the plates are properly oriented, punch a hole through the foil cover using a pipette - into the well you want the DNA from. Don't remove foil cover since it might...')
 
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1. Making sure the plates are properly oriented, punch a hole through the foil cover using a pipette - into the well you want the DNA from. Don't remove foil cover since it might pose the threat of cross-contamination between wells.
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MIT sends DNA kits with the biological parts needed to construct the required bacteria.
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There is a specific procedure for 'taking out' the dry DNA from the kit. The DNA is stored in these pits in the box called 'well' and below is the standard procedure of extracting the required DNA:
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Mking sure the plates are properly oriented, punch a hole through the foil cover using a pipette - into the well you want the DNA from. Don't remove foil cover since it might pose the threat of cross-contamination between wells.
  
 
2. Add 15uL of deionized water - diH2O
 
2. Add 15uL of deionized water - diH2O
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<gallery>
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File:IMG_5881_-Desktop_Resolution-.JPG|The Dry DNA kit
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File:DSC_8084.jpg|Extracting the dry DNA from the wells.
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</gallery>

Revision as of 08:29, 24 June 2009


MIT sends DNA kits with the biological parts needed to construct the required bacteria.

There is a specific procedure for 'taking out' the dry DNA from the kit. The DNA is stored in these pits in the box called 'well' and below is the standard procedure of extracting the required DNA:

Mking sure the plates are properly oriented, punch a hole through the foil cover using a pipette - into the well you want the DNA from. Don't remove foil cover since it might pose the threat of cross-contamination between wells.

2. Add 15uL of deionized water - diH2O