Preparing the pBAD and Lysis plasmids for gel electrophoresis

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  1. Take 1.5ml of the culture in an epenndorff tube.
  2. Centrifuge the culture at 13200 rpm for 3 minutes, making sure the centrifuge is balanced. The centrifugal force created in the centrifuge forces the bacteria in the solution to collect in a pellet at the bottom of the eppendorff tube.
  3. The liquid around the collected pellet, called the supernatant can be discarded.
  4. Dissolve the pellet in 150µl ALS I