Difference between revisions of "Our Step-wise Process"
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'''Step 6 - [[Digesting the DNA]]''' | '''Step 6 - [[Digesting the DNA]]''' | ||
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+ | '''Step 7 - [[Gel Electrophoresis]]''' |
Latest revision as of 19:29, 16 June 2009
Step 1 - Taking dry DNA from wells
Step 2 - Transforming competent cells. Competent cells are those cells which have the ability to take up extracellular/naked DNA from its environment.
Step 3 - Picking a single colony.
Step 4 - Inoculainge broth with Ampicillin-R (an antibiotic) and letting it grow for 18 hours.
Step 5 - Using the resulting culture for mini-prep. - a process used to purify plasmids and yields clean, usable DNA.
Step 6 - Digesting the DNA
Step 7 - Gel Electrophoresis