Difference between revisions of "Jugaad PCR Thermocyclers"

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Here we go;<br>
 
Here we go;<br>
 
[[File:pcr_laserhousing.jpg|none|400px|]]
 
[[File:pcr_laserhousing.jpg|none|400px|]]
 +
And the one pdf with all the parts as of November 2012:<br>
 
[[File:pcr_laserfiles.pdf|none|400px|]]
 
[[File:pcr_laserfiles.pdf|none|400px|]]
 
<br>
 
<br>
Line 52: Line 53:
 
- Arduino ok, SMD driver board with AD converter, connectors, feasible. Will try to simplify the layout to make it single-side DIY. What are the big resistors for? Some kind of heating? Have to figure out.<br>
 
- Arduino ok, SMD driver board with AD converter, connectors, feasible. Will try to simplify the layout to make it single-side DIY. What are the big resistors for? Some kind of heating? Have to figure out.<br>
 
- LCD Display - standard, anything goes, 4 Lines are nice.<br>
 
- LCD Display - standard, anything goes, 4 Lines are nice.<br>
 +
<br>
 +
Now one tricky part is the heatblock and the peltier element. For those we have two nice hacks:
 +
1. A peltier element with the exact right dimensions and with some luck even the rough thermo-block can be found in one of those little electric fridges:<br>
 +
[[File:pcr_fridges.jpg|none|400px|]]<br>
 +
Found those in electronic waste.<br>
 +
[[File:pcr_extraction.jpg|none|400px|]]
 +
Unscrew the blower and the cooler and you will find the peltier element.
 +
[[File:pcr_heat_parts.jpg|none|400px|]]
 +
All you need, a aluminium heat-block (thing the original OpenPCR heat-block is cooper and then chromed, right?), peltier element...<br>
 
<br>
 
<br>

Revision as of 10:44, 26 November 2012

Ideas

Coconut PCR :-)

Links

DIY thermocycler

some discussion about the light bulb and fan thermocycler in DIYbio maillits.

referring to an indian research group

and remade during the FutureLabCamp2011 [here] by Russell Durrett, see his blog on PCR

cathal harvey showed me the hot airgun version with relay, no posts found yet on indie biotech

open hardware thermocycler

The classic OpenPCR kit to order, assemble and hack by openPCR.com

personal PCR on an arduino shield (only 2 tubes) by cofactorbio personalPCR


OpenPCR in the wild

Attempt to build an OpenPCR just from the desctription on the website OpenPCR without a kit or any supplied parts.

First and easy thing is to go up to a FabLab and laser cut the wood parts for the housing. Need to get the files from the website and have the most recent version of SolidWorks to extract the drawings...

Here we go;

Pcr laserhousing.jpg

And the one pdf with all the parts as of November 2012:
File:Pcr laserfiles.pdf
Got some additional information from Josh from OpenPCR: The downloadable files are the more recent versions of our design.

And:

The OpenPCR BOM can be found here:
https://docs.google.com/a/snowrise.com/spreadsheet/ccc?key=0AiaWH3PL_9CndG1GbmtSZ1I3aGdkQkE4YVhwZ3o5c1E#gid=0
Good to know.
What else do we need?
- The PC-Cooler with 90° heat pipe is easy to get for around 20 euro.
- The nice and small power supply that fits into the housing is not so easy. It is a "Flex 1u PC Power Supply 200 W ATX. Bigger (standard size is more easy and should do, just won't fit into the housing
- Arduino ok, SMD driver board with AD converter, connectors, feasible. Will try to simplify the layout to make it single-side DIY. What are the big resistors for? Some kind of heating? Have to figure out.
- LCD Display - standard, anything goes, 4 Lines are nice.

Now one tricky part is the heatblock and the peltier element. For those we have two nice hacks: 1. A peltier element with the exact right dimensions and with some luck even the rough thermo-block can be found in one of those little electric fridges:

Pcr fridges.jpg

Found those in electronic waste.

Pcr extraction.jpg

Unscrew the blower and the cooler and you will find the peltier element.

Pcr heat parts.jpg

All you need, a aluminium heat-block (thing the original OpenPCR heat-block is cooper and then chromed, right?), peltier element...