Difference between revisions of "GlobalLAMP-Yaoundé"

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Motivated by the work in Paris and by the good results of the crude camera prototype Urs has worked hard at GaudiLabs to develop the idea into a new device called qPocketPCR. Based on the knowledge of the successful [PocketPCR [https://gaudi.ch/PocketPCR/]] machine the new device reads a tiny camera through an ESP32 to read the fluorescence from all 8 tubes with a high sensitivity image taken from the side. The device then can show the real image of the fluorescent tubes on the LCD display and calculate the intensity for each tube. The device is very compact and can run from USB-C PD.  <br><br>
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Motivated by the work in Paris and by the good results of the crude camera prototype Urs has worked hard at GaudiLabs to develop the idea into a new device called qPocketPCR. Based on the knowledge of the successful [PocketPCR https://gaudi.ch/PocketPCR] machine the new device reads a tiny camera through an ESP32 to read the fluorescence from all 8 tubes with a high sensitivity image taken from the side. The device then can show the real image of the fluorescent tubes on the LCD display and calculate the intensity for each tube. The device is very compact and can run from USB-C PD.  <br><br>
  
  

Revision as of 10:08, 18 October 2022

GloablLAMPAfrica.jpg

Collaborative Development of Isothermal Amplification & Devices
September 24. - October 5., 2022 Yaoundé Cameroon

Scope of the Project

This is the second phase of the project. For the first phase we met in Paris at the Learning Planet Institute GlobalLAMP-Paris to develop ideas, device prototypes and made first comparative measurements. In this second phase we are working on producing the devices in medium-scale and focus more on the applicability of the developed equipment in a real-world scenario. Two devices, the open source qLAMP - developed by Francisco Quero and the qPocketPCR developed by Urs Gaudenz at GaudiLabs have been developed further to a near production ready level. We will now establish a production chain to build the first batch of devices and the necessary reaction mixes that later on will be used to test the system in the field. To get better insight into potential applications and the use in a real world scenario we gathered at MboaLab in Yaoundé, Cameroon to test systems and meet local experts from the field.

Devices


Different devices with varying technical features and advantages are developed and produced in parallel. The diversity of solutions allows us to compare and learn from each other while serving different needs, applications and markets.
openQLAMP
M3O1rg3AWaQuwGjIblYhUJaidfpfSaMq81v38Ajo.jpeg
The openQLAMP machine was developed further with many important technical improvements such as flexible PCB heater with several heating sections and new SMD excitation LED board for efficient production. Also the software part was improved based on experiences from first tests (eg. pre-heating of the heated lid etc).

qPocketPCR
QPocketPCR Inside.jpg IMG 20220921 145833 edit 94823849436572.jpg
Motivated by the work in Paris and by the good results of the crude camera prototype Urs has worked hard at GaudiLabs to develop the idea into a new device called qPocketPCR. Based on the knowledge of the successful [PocketPCR https://gaudi.ch/PocketPCR] machine the new device reads a tiny camera through an ESP32 to read the fluorescence from all 8 tubes with a high sensitivity image taken from the side. The device then can show the real image of the fluorescent tubes on the LCD display and calculate the intensity for each tube. The device is very compact and can run from USB-C PD.


Quantitative real-time DNA amplification is at the heart of either diagnosing infectious disease or any section of biology research. Use of these devices has been limited due its cost and availability, therefore, refuting to elaborate and end-point methods like gel-electrophoresis. This project will change accessibility and affordability of this device to specially low-income countries either for POC or teaching purposes. Furthermore, the core of the project includes researchers and collaborators from diverse geographical backgrounds, professional expertise and socioeconomic origins, either at the development stage or at the end-user level at the next phases.

Locations