Difference between revisions of "DIY NanoDrop"

From Hackteria Wiki
Jump to: navigation, search
Line 13: Line 13:
  
 
<br>
 
<br>
 +
Now the NanoDrop principle seems to be patented (patent US6628382 and US6809826)
 
== LED-Photosensor  ==
 
== LED-Photosensor  ==
  
Line 23: Line 24:
  
  
=== LED-Spectrometer ===
+
== LED-Spectrometer ==
  
 
[[File:NanoDropConcept2.jpg|320px]]
 
[[File:NanoDropConcept2.jpg|320px]]

Revision as of 10:46, 17 February 2014

DIYNanoDrop.jpg

Attempt to build DIY Microvolume Spectrophotometers or Fluorospectrometers to quantitate micro-volumes of sample. The NanoDrop products are very popular in scientific labs. The measurement principle of holding a drop between two points - the light source and the sensor - allows to measure samples as small as 0.5µL up to 2µL micro volume without a cuvette. The simple basic principle should be perfect for DIY lab equipment. Let's try.
To start simple, two approaches are tested, one using an LED and a photo sensor. The second, more advanced, using a DIY spectrometer.
Possible applications:

- Turbidity measurement
- Spectral data and purity analysis
- Measure nucleic acid, protein and colorimetric assays
- Microvolume fluorescence measurements


Now the NanoDrop principle seems to be patented (patent US6628382 and US6809826)

LED-Photosensor

320px

High performance LEDs can replace expensive light source and filters for excitation.



LED-Spectrometer

320px