Difference between revisions of "DIY NanoDrop"

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Attempt to build DIY Microvolume Spectrophotometers or Fluorospectrometers to quantitate micro-volumes of sample.
 
Attempt to build DIY Microvolume Spectrophotometers or Fluorospectrometers to quantitate micro-volumes of sample.
The NanoDrop products are very popular in scientific labs. The measurement principle of holding a drop between two points - the light source and the sensor - allows to measure samples as small as 0.5µL up to 2µL micro volume without a cuvette. The basically simple principle should be perfect for DIY lab equipment. <br>
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The NanoDrop products are very popular in scientific labs. The measurement principle of holding a drop between two points - the light source and the sensor - allows to measure samples as small as 0.5µL up to 2µL micro volume without a cuvette. The simple basic principle should be perfect for DIY lab equipment. Let's try.<br>
To start simple, two approaches are tested, one using an LED and a photo sensor. The second, more advanced, using a DIY spectrometer.
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To start simple, two approaches are tested, one using an LED and a photo sensor. The second, more advanced, using a DIY spectrometer.<br>
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Possible applications:<br>
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<br>
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- Turbidity measurement<br>
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- Spectral data and purity analysis<br>
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- Measure nucleic acid, protein and colorimetric assays<br>
 +
- Microvolume fluorescence measurements<br>
  
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<br>
 
== LED-Photosensor  ==
 
== LED-Photosensor  ==
  
  
 
[[File:DIY_NanoDropConcept1.jpg|320px]]
 
[[File:DIY_NanoDropConcept1.jpg|320px]]
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High performance LEDs can replace expensive light source and filters for excitation.
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Revision as of 10:42, 17 February 2014

DIYNanoDrop.jpg

Attempt to build DIY Microvolume Spectrophotometers or Fluorospectrometers to quantitate micro-volumes of sample. The NanoDrop products are very popular in scientific labs. The measurement principle of holding a drop between two points - the light source and the sensor - allows to measure samples as small as 0.5µL up to 2µL micro volume without a cuvette. The simple basic principle should be perfect for DIY lab equipment. Let's try.
To start simple, two approaches are tested, one using an LED and a photo sensor. The second, more advanced, using a DIY spectrometer.
Possible applications:

- Turbidity measurement
- Spectral data and purity analysis
- Measure nucleic acid, protein and colorimetric assays
- Microvolume fluorescence measurements


LED-Photosensor

320px

High performance LEDs can replace expensive light source and filters for excitation.



LED-Spectrometer

320px