Difference between revisions of "DIY NanoDrop"

From Hackteria Wiki
Jump to: navigation, search
(LED-Spectrometer)
Line 1: Line 1:
  
[[File:DIYNanoDrop.jpg|440px]]
+
 
  
 
Attempt to build DIY Microvolume Spectrophotometers or Fluorospectrometers to quantitate micro-volumes of sample.
 
Attempt to build DIY Microvolume Spectrophotometers or Fluorospectrometers to quantitate micro-volumes of sample.
 
The NanoDrop products are very popular in scientific labs. The measurement principle of holding a drop between two points - the light source and the sensor - allows to measure samples as small as 0.5µL up to 2µL micro volume without a cuvette. The simple basic principle should be perfect for DIY lab equipment. Let's try.<br>
 
The NanoDrop products are very popular in scientific labs. The measurement principle of holding a drop between two points - the light source and the sensor - allows to measure samples as small as 0.5µL up to 2µL micro volume without a cuvette. The simple basic principle should be perfect for DIY lab equipment. Let's try.<br>
 
To start simple, two approaches are tested, one using an LED and a photo sensor. The second, more advanced, using a DIY spectrometer.<br>
 
To start simple, two approaches are tested, one using an LED and a photo sensor. The second, more advanced, using a DIY spectrometer.<br>
<br>
 
[[File:DIY_NanoDropProto1.jpg|210px]]<br>
 
 
First prototype.<br>
 
 
<br>
 
<br>
  
Line 17: Line 13:
 
- Measure nucleic acid, protein and colorimetric assays<br>
 
- Measure nucleic acid, protein and colorimetric assays<br>
 
- Microvolume fluorescence measurements<br>
 
- Microvolume fluorescence measurements<br>
 +
 +
 +
First prototype.<br>
 +
 +
[[File:DIY_NanoDropProto1.jpg|210px]]<br>
 +
 +
Prototype of LED-Photosensor Version
 +
[[File:DIYNanoDrop.jpg|440px]]
  
 
<br>
 
<br>

Revision as of 15:19, 14 October 2014


Attempt to build DIY Microvolume Spectrophotometers or Fluorospectrometers to quantitate micro-volumes of sample. The NanoDrop products are very popular in scientific labs. The measurement principle of holding a drop between two points - the light source and the sensor - allows to measure samples as small as 0.5µL up to 2µL micro volume without a cuvette. The simple basic principle should be perfect for DIY lab equipment. Let's try.
To start simple, two approaches are tested, one using an LED and a photo sensor. The second, more advanced, using a DIY spectrometer.

Possible applications:

- Turbidity measurement
- Spectral data and purity analysis
- Measure nucleic acid, protein and colorimetric assays
- Microvolume fluorescence measurements


First prototype.

DIY NanoDropProto1.jpg

Prototype of LED-Photosensor Version DIYNanoDrop.jpg


Now the NanoDrop principle seems to be patented (patent US6628382 and US6809826). However there are many similar products on the market under the term of "Microvolume Spectrophotometers"...? Doesn't matter anyways if you just build one for your own use.

DIY NanoDropPatent1.pngDIY NanoDropPatent2.png

DIY NanoDropTechnicalDrawing1.png


LED-Photosensor

A flat head LED and a flat head photo diode are put together, a drop of liquid in between - this is it. Maybe add a screw to set the distance between the light source and the sensor. A small arduino can measure the light sensor and send the data to the computer via USB.

DIY NanoDropConceptLED.png

High performance LEDs can replace expensive light source and filters for excitation.

LED-Spectrometer

More advanced version based on a DIY spectrometer using a DVD-R optical diffraction grating and a USB webcam as described by PublicLab. Use the excellent online software workbench to analyse data - http://spectralworkbench.org/ .


NanoDropConceptSpectrometer2.png

Links

http://www.google.com/patents/US6628382
http://www.google.com/patents/US6809826
http://www.google.com/search?q=Microvolume+Spectrophotometers&source=lnms&tbm=isch