Difference between revisions of "DIY Laser tweezer, cell trap, oligo synthesis"

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[[File:trap_Lasersoldering.jpg|none|400px|]]<br>
 
[[File:trap_Lasersoldering.jpg|none|400px|]]<br>
 
[[File:trap_Laser_pinout.jpg|none|400px|]]<br>
 
[[File:trap_Laser_pinout.jpg|none|400px|]]<br>
 +
 +
== Powering the Laser ==
 +
Started rebuilding the optical tweezers.<br>
 +
<br>
 +
First Notes:<br>
 +
- The schematic in the papaer to power the laser uses an "unusual" pinout of the LM317. Refer to the schematic below rather.<br>
 +
- The suggested current of 350mA for a DVD laser diode is at the upper limit of what most of these laser can take (without extra cooling). So go slowly and expect to blow up your diode at 350 mA (what happend to me)<br>
 +
- Actualy the discription of the diagram in the paper says 420 mA. When I recalculate the current (with R=4 Ohm and UAdj=1.25V (I=UAdj/R) I get a current of only 312 mA. This is probably a good value :-)<br>
 +
- Generaly laser diodes are quite delicate. When they suddenly get less bright then they are in the so called "LED" mode and kaput.<br>
 +
<br>
 +
<br>
 +
[[File:trap_schema.jpg|none|500px|]]<br>
 +
R=4 Ohm (in total)
 +
Pot= 100 Ohm or less.<br>
 +
[[File:trap_board.jpg|none|500px|]]<br>
 +
<br>
 +
<br>
 +
  
 
== Mechanical Setup ==
 
== Mechanical Setup ==
Line 20: Line 38:
 
[[File:trap_Beads15.jpg|none|300px|]]<br>
 
[[File:trap_Beads15.jpg|none|300px|]]<br>
  
Started rebuilding the optical tweezers.<br>
 
<br>
 
First Notes:<br>
 
- The schematic in the papaer to power the laser uses an "unusual" pinout of the LM317. Refer to the schematic below rather.<br>
 
- The suggested current of 350mA for a DVD laser diode is at the upper limit of what most of these laser can take (without extra cooling). So go slowly and expect to blow up your diode at 350 mA (what happend to me)<br>
 
- Actualy the discription of the diagram in the paper says 420 mA. When I recalculate the current (with R=4 Ohm and UAdj=1.25V (I=UAdj/R) I get a current of only 312 mA. This is probably a good value :-)<br>
 
- Generaly laser diodes are quite delicate. When they suddenly get less bright then they are in the so called "LED" mode and kaput.<br>
 
<br>
 
<br>
 
[[File:trap_schema.jpg|none|500px|]]<br>
 
R=4 Ohm (in total)
 
Pot= 100 Ohm or less.
 
<br>
 
<br>
 
  
  

Revision as of 11:26, 2 October 2012

Trap Sprint.jpg

Harvesting the Laser

Trap Laser.jpg

Trap Lasersoldering.jpg

Trap Laser pinout.jpg

Powering the Laser

Started rebuilding the optical tweezers.

First Notes:
- The schematic in the papaer to power the laser uses an "unusual" pinout of the LM317. Refer to the schematic below rather.
- The suggested current of 350mA for a DVD laser diode is at the upper limit of what most of these laser can take (without extra cooling). So go slowly and expect to blow up your diode at 350 mA (what happend to me)
- Actualy the discription of the diagram in the paper says 420 mA. When I recalculate the current (with R=4 Ohm and UAdj=1.25V (I=UAdj/R) I get a current of only 312 mA. This is probably a good value :-)
- Generaly laser diodes are quite delicate. When they suddenly get less bright then they are in the so called "LED" mode and kaput.


Trap schema.jpg

R=4 Ohm (in total) Pot= 100 Ohm or less.

Trap board.jpg




Mechanical Setup

Trap Setup def.jpg

Trap Setup1.jpg

Trap PictureSetup1.jpg

Trap Setup2.jpg

Trap Setup3.jpg

The objects to move arround





Single-cell isolation using a DVD optical pickup
A. Kasukurti, M. Potcoava, S.A. Desai, C. Eggleton, and D. W. M. Marr1,*
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3169297/

Optical tweezers directed one-bead one-sequence synthesis of
oligonucleotides†
http://www.ncbi.nlm.nih.gov/pubmed/21445444

Interferometry with optical pickups.
Quercioli F, Tiribilli B, Bartoli A.
http://www.ncbi.nlm.nih.gov/pubmed/18073818

A CHEAP 2D FLUORESCENCE DETECTION SYSTEM
FOR μM-SIZED BEADS ON-CHIP
Loes I. Segerink*, Maarten J. Koster, Ad J. Sprenkels, Istvan Vermes and Albert van den Berg

A new DNA chip detection mechanism using optical pick-up actuators
Kyung-Ho Kim, Seung-Yop Lee, Sookyung Kim, Seung-Hwan Lee and Seong-Gab Jeong
http://www.springerlink.com/content/084133p3n5mk1411/