Difference between revisions of "DIY $25 Gel Box"

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</div>
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The Gel-Electrophoresis-Machine is constructed by creating a chamber that holds an electrolyte(salt water) that allows the conduction of electricity. A potential would be applied across it. Within, an agarose gel is placed and this also experiences the potential applied on the ends of the chamber.
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In the gel are wells containing substances of interest that have a know charge. Potential is applied in order to pull the substance through the agarose gel. It is apparent that the smaller molecules would travel further through the gel than the larger molecules. Thus, with a ladder-a set of molecules with known DNA lengths or molecule size, comparison would allow us to determine the length/size of the molecule of interest.
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We constructed a functional Gel Electrophoresis Chamber in a span of a day using :
 
We constructed a functional Gel Electrophoresis Chamber in a span of a day using :
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Materials:
 
Materials:
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7.Heat shrinking tubing, one black and one red
 
7.Heat shrinking tubing, one black and one red
  
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'''The Materials used:'''
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&lt;gallery&gt;
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<gallery>
 
<gallery>
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File:Materials.jpg |Different Dimensions of Plexiglass
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File:Electricalplugs.JPG|Banana Jacks
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File:Banana_plugs.JPG|Banana Plugs
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File:TEFLON.jpg|Teflon Piece
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File:wires.jpg|The Electrical Wires
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File:Adhesiveetc.jpg|The Adhesive etc
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</gallery>
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&lt;/gallery&gt;
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'''The Building Process:'''
  
File:Materials.jpg
 
File:Electricalplugs.jpg
 
  
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&lt;gallery&gt;
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<gallery>
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File:Process1.jpg|Building the chamber
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File:Process2.jpg
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File:Stage2.jpg
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File:Teflon_Cutting.jpg|Cutting the teflon
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File:Cut_Teflon.jpg|The Cut Piece of Teflon
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File:Process3.jpg
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File:The_Unit.jpg|The Constructed Unit
 
</gallery>
 
</gallery>
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&lt;/gallery&gt;
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== June 1st ==
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'''Gel Electrophoresis using Food Dyes:'''
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Once we had constructed the Electrophoresis machine, we put it to test. We took 3 food dyes and created their respective solutions. We placed them in the wells and ran the machine for close to 50 minutes. Unfortunately, our samples flowed in the wrong direction. Since we were unable to determine which dye had which charge and which wire was +ve or -ve, we had to improvise. We placed the wells in the center of the gel and decided to let the dyes flow in either direction.
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&lt;gallery&gt;
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<gallery>
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File:DSC_8011.jpg|
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File:DSC_8012.jpg|
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File:DSC_8017.jpg|
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File:DSC_8021.jpg|
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File:DSC_8027.jpg|
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File:DSC_8030.jpg|
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File:DSC_8032.jpg|
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File:DSC_8033.jpg|
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File:DSC_8034.jpg|
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File:DSC_8035.jpg|
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File:DSC_8037.jpg|
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File:DSC_8039.JPG|
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File:DSC_8040.jpg|
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File:DSC_8041.jpg|
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File:DSC_8042.jpg|
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File:DSC_8046.jpg|
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File:DSC_8048.jpg|
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File:DSC_8050.jpg|
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</gallery>
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&lt;/gallery&gt;
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== Useful Links ==
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How to make DIY Gel:<br>
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http://learn.genetics.utah.edu/content/labs/gel/electrophoresis/<br>
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<br>
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Excellent DIY instruction:<br>
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teach.genetics.utah.edu/content/build_gel_box.pdf<br>
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<br>
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How to prepare Gel:<br>
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http://m.instructables.com/id/How-to-Prepare-an-Electrophoresis-Argarose-Gel/step3/Cast-the-Gel/<br>
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<br>
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mac giver staaayle:
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http://www.scq.ubc.ca/the-macgyver-project-genomic-dna-extraction-and-gel-electrophoresis-experiments-using-everyday-materials/

Latest revision as of 08:16, 30 March 2017

</div> The Gel-Electrophoresis-Machine is constructed by creating a chamber that holds an electrolyte(salt water) that allows the conduction of electricity. A potential would be applied across it. Within, an agarose gel is placed and this also experiences the potential applied on the ends of the chamber. In the gel are wells containing substances of interest that have a know charge. Potential is applied in order to pull the substance through the agarose gel. It is apparent that the smaller molecules would travel further through the gel than the larger molecules. Thus, with a ladder-a set of molecules with known DNA lengths or molecule size, comparison would allow us to determine the length/size of the molecule of interest.

We constructed a functional Gel Electrophoresis Chamber in a span of a day using :


Materials:

1.Plexiglass

2.Teflon

3.Banana Jacks

4.Banana Plugs

5.Black and red Test Lead Wire

6.Copper wire

7.Heat shrinking tubing, one black and one red

The Materials used: <gallery>

</gallery>

The Building Process:


<gallery>

</gallery>

June 1st

Gel Electrophoresis using Food Dyes:

Once we had constructed the Electrophoresis machine, we put it to test. We took 3 food dyes and created their respective solutions. We placed them in the wells and ran the machine for close to 50 minutes. Unfortunately, our samples flowed in the wrong direction. Since we were unable to determine which dye had which charge and which wire was +ve or -ve, we had to improvise. We placed the wells in the center of the gel and decided to let the dyes flow in either direction.


<gallery>

</gallery>

Useful Links

How to make DIY Gel:
http://learn.genetics.utah.edu/content/labs/gel/electrophoresis/

Excellent DIY instruction:
teach.genetics.utah.edu/content/build_gel_box.pdf

How to prepare Gel:
http://m.instructables.com/id/How-to-Prepare-an-Electrophoresis-Argarose-Gel/step3/Cast-the-Gel/

mac giver staaayle:

http://www.scq.ubc.ca/the-macgyver-project-genomic-dna-extraction-and-gel-electrophoresis-experiments-using-everyday-materials/