Difference between revisions of "Back to Bombyx"
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==Methods== | ==Methods== | ||
===Obtaining Silk Protein=== | ===Obtaining Silk Protein=== | ||
| + | # Degumming or removal of sericin - 5g/L sodium carbonate, pH, T, 1h (for Bombyx). | ||
| + | # Dissolution of fibroin | ||
[https://drive.google.com/file/d/0BzF8kxTIehaoV2Y0VGZvUUFEWTg/view Step-by-step documentation] from HTGAA 2015 from silklab | [https://drive.google.com/file/d/0BzF8kxTIehaoV2Y0VGZvUUFEWTg/view Step-by-step documentation] from HTGAA 2015 from silklab | ||
| + | ===LiBr or not=== | ||
| + | Lithium Bromide (LiBr), while one can source it online, as a non-institutional person, still can be hard to find.<br> | ||
| + | Brief summary of reading some papers is that LiBr is the best for degummed fibers of silk cocoons, where nearly 100% can be solubilized in LiBr, compared to 30% in CaCl2, or 50% NaSCN (sodium thiocyanate) (numbers taken from [http://scialert.net/fulltext/?doi=pjbs.2013.1199.1203&org=11 Addis and Raina 2013]).<br> | ||
| + | A [https://www.researchgate.net/post/Could_you_suggest_me_a_solvent_to_solubilize_silk_fibroin Researchgate thread] mentioned "2.5wt% silk fibroin in 28% ammonium aqueous solution by magnetic stirring." | ||
==Sourcing== | ==Sourcing== | ||
Revision as of 19:42, 3 April 2017
Silk Worms are Back!
Bombyx mori is the domesticated silk worm bred for silk production.
Contents
Methods
Obtaining Silk Protein
- Degumming or removal of sericin - 5g/L sodium carbonate, pH, T, 1h (for Bombyx).
- Dissolution of fibroin
Step-by-step documentation from HTGAA 2015 from silklab
LiBr or not
Lithium Bromide (LiBr), while one can source it online, as a non-institutional person, still can be hard to find.
Brief summary of reading some papers is that LiBr is the best for degummed fibers of silk cocoons, where nearly 100% can be solubilized in LiBr, compared to 30% in CaCl2, or 50% NaSCN (sodium thiocyanate) (numbers taken from Addis and Raina 2013).
A Researchgate thread mentioned "2.5wt% silk fibroin in 28% ammonium aqueous solution by magnetic stirring."
Sourcing
CH
IN
- Department of Sericulture Government of Karnataka
- Silkworm Seed Production Centre, Government Office Bengaluru, Karnataka
- National Silkworm Seed Organization, Agricultural Seed Store, Bengaluru, Karnataka
- Silk Worm Breeding Unit, Bengaluru, Karnataka
- Sri Lingeshwara Silk Worm Seed Product, Kamenahalli, Karnataka
References
About the Silk Worm
- Microscopic Studies of Bombyx mori David Walker, UK
Material Properties and Methods
- Polyol-Silk Bioink Formulations as Two-Part Room-Temperature Curable Materials for 3D Printing ACS Biomater. Sci. Eng., 2015, 1 (9), pp 780–788 DOI: 10.1021/acsbiomaterials.5b00160: The researchers combined silk proteins, which are biocompatible, and glycerol, a non-toxic sugar alcohol commonly found in food and pharmaceutical products. The resulting ink was clear, flexible, stable in water, and didn’t require any processing methods.
- Pdf of Materials fabrication from Bombyx mori silk fibroin, Nat Protoc. 2011 Sep 22;6(10):1612-31. doi: 10.1038/nprot.2011.379.
- HTGAA 2015 Lucern documentation on bioprinting
Molecular Biology
- SilkDB a draft genome sequence for the silkworm B. mori, by the Beijing Genomics Institute (BGI)
- SilkDB for the silkworm, Bombyx mori from Scripps and , genomic data including functional annotation of genes, gene products and chromosomal mapping, extensive biological information such as microarray expression data, ESTs and corresponding references
- WildSilkbase BLAST searchable catalogue of expressed sequence tags (ESTs) generated from several tissues of wild silkmoths at different developmental stages, Center for DNA Fingerprinting and Diagnosis, Hydrabad India
- Insect Proteome Database, Silkworm
- Table 2.2 Genomics and Functional Genomics Resources Molecular Biology and Genetics of the Lepidoptera edited by Marian R. Goldsmith, Frantisek Marec, CRC Press ISBN 1420060201, 9781420060201.
- Key enzymes and proteins of crop insects as candidate for RNAi based gene silencing Kola et al. Department of Biotechnology, Directorate of Rice Research, ICAR-Indian Institute of Rice Research, Hyderabad, India. Front. Physiol., 22 April 2015 | https://doi.org/10.3389/fphys.2015.00119
