Difference between revisions of "A Brief Description of What We Will Do"
From Hackteria Wiki
Line 1: | Line 1: | ||
− | '''Step 1 - Taking dry DNA from wells''' | + | '''[[Step 1 - Taking dry DNA from wells]]''' |
− | |||
− | |||
'''[[Step 2 - Transforming competent cells.]]''' | '''[[Step 2 - Transforming competent cells.]]''' | ||
''Competent cells are those cells which have the ability to take up extracellular/naked DNA from its environment.'' | ''Competent cells are those cells which have the ability to take up extracellular/naked DNA from its environment.'' |
Revision as of 16:44, 16 June 2009
Step 1 - Taking dry DNA from wells
Step 2 - Transforming competent cells.
Competent cells are those cells which have the ability to take up extracellular/naked DNA from its environment.
Step 3 - Pick a single colony.
Step 4 - Inoculate broth with Ampicillin-R (an antibiotic) and let it grow for 18 hours.
Step 5 - Use the resulting culture for mini-prep. - a process used to purify plasmids and yields clean, usable DNA.