D.nft also known as XMT-CYB, MT-CYBo
Synopsis: Under expression of D.nft disrupts female fertility, causes general debility and makes adverse effects on health. Mitochondrial Cytochrome B Stops inflation with free radicals. Over expression may be Anti artherosclerosis and explain effects on migrants stuck in Chinese and Bangladeshi Xinjiang Uyghur camps with their origin mutant MT-CYBo X. If Severe Obesity, give Benzo Barbs and remain clinically silent, you homoplasmic carriers! Mecha-spank.
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D.nft GOSPHA Basic Information Name of Creative Germline Transgenic Human Genome Alternatives Construct: D.nft XMT-CYB, MT-CYBo
Team Names: Ionian Pharma, HioHubGitGutter
Name of Gene of Study: cytochrome b
How do you usually express yourself: Other
What Organism(s) is the Gene Found In? : conical sea squirt (Lat. Aplidium conicum), human
Where in the Organism(s) is the Gene Expressed? : Mitochondria
What Does the Gene Do? What is the Action or Effect of the Gene? Mutation can cause health issues in sheep and effects female reproduction of Ghungroo pig. Mutation in Cytochrome B gene generally causes debility and adverse effects on health of sheep. Mutations in cytochrome B gene effects female reproduction of Ghungroo pigs.
Scientist involved in cytochrome b research: Aruna Pal, West Bengal University of Animal and Fishery Sciences, 37, K.B. Sarani, Kolkata 37, India.
Method(s) of Transgenesis,:
recombinant viruses (sometimes called biological nanoparticles or viral vectors), for instance: adeno-associated viruses (AAVs) (relative of herpes), oncoretroviral, lentiviral retroviruses (i.e. human immunodeficiency virus (HIV), which causes AIDS), adenoviruses, herpes simplex, vaccinia (pox), and human foamy virus.; microinjection; Photoporation (laser); aerosol/colonic: on or in mucosal surfaces, such as up the nasal and inhaled into lung mucosa or enema/pneumatic (jet) injection
Safe Harbor Landing Pads:
human SHS AAVS1; "Safe" Mongr X: address on the X chromosome in Base Pairs (bp) chrX: 79,674,328–79,674,347 Sequence AAAATGTCATAAGgCAGTTT 3 out of 8 on the 'safe' harbor scale, site ID 321 from Table 2. Location and scoring of canonical and newly identified potential human safe harbor sites, New Human Chromosomal Sites with ‘‘Safe Harbor’’ Potential for Targeted Transgene Insertion, Hum Gene Ther . 2019 Jul;30(7):814-828. doi: 10.1089/hum.2018.169. Epub 2019 Mar 28.
Light Shock, Optogenetically Refined Wavelengths TBA; Synth Cassette-optimized gene control with new inducer(s) of choice, i.e. the sound of frogs or popular condiments like mustard or sriracha mayonnaise, preventing off-target expression and reducing potential side effects of the treatment.; UBC Human ubiquitin C promoter, Ubiquitous in many ways.
Resistance to persuasion, If you’re expressing a gene that may be toxic, it’s important that your inducible promoter not be too leaky. Trangenic humans with resistance to persuasion are known for their low leakiness. ; Gene Ween, tunable expression levels; Brainbow-2: random expression of different XFP ratios and subsequently causing different cells to exhibit a variety of colorful hues.; tattletale (TTLTL) DNA that codes for reporter protein molecules; specific membrane somatostatin receptors (hSSTrs) + DMT
Homo sapiens mitochondrion, complete genome NCBI Reference Sequence: NC_012920.1 GenBank Graphics >NC_012920.1:14747-15887 Homo sapiens mitochondrion, complete genome ATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCTCCCCACCC CATCCAACATCTCCGCATGATGAAACTTCGGCTCACTCCTTGGCGCCTGCCTGATCCTCCAAATCACCAC AGGACTATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAATCGCCCACATCACT CGAGACGTAAATTATGGCTGAATCATCCGCTACCTTCACGCCAATGGCGCCTCAATATTCTTTATCTGCC TCTTCCTACACATCGGGCGAGGCCTATATTACGGATCATTTCTCTACTCAGAAACCTGAAACATCGGCAT TATCCTCCTGCTTGCAACTATAGCAACAGCCTTCATAGGCTATGTCCTCCCGTGAGGCCAAATATCATTC TGAGGGGCCACAGTAATTACAAACTTACTATCCGCCATCCCATACATTGGGACAGACCTAGTTCAATGAA TCTGAGGAGGCTACTCAGTAGACAGTCCCACCCTCACACGATTCTTTACCTTTCACTTCATCTTGCCCTT CATTATTGCAGCCCTAGCAACACTCCACCTCCTATTCTTGCACGAAACGGGATCAAACAACCCCCTAGGA ATCACCTCCCATTCCGATAAAATCACCTTCCACCCTTACTACACAATCAAAGACGCCCTCGGCTTACTTC TCTTCCTTCTCTCCTTAATGACATTAACACTATTCTCACCAGACCTCCTAGGCGACCCAGACAATTATAC CCTAGCCAACCCCTTAAACACCCCTCCCCACATCAAGCCCGAATGATATTTCCTATTCGCCTACACAATT CTCCGATCCGTCCCTAACAAACTAGGAGGCGTCCTTGCCCTATTACTATCCATCCTCATCCTAGCAATAA TCCCCATCCTCCATATATCCAAACAACAAAGCATAATATTTCGCCCACTAAGCCAATCACTTTATTGACT CCTAGCCGCAGACCTCCTCATTCTAACCTGAATCGGAGGACAACCAGTAAGCTACCCTTTTACCATCATT GGACAAGTAGCATCCGTACTATACTTCACAACAATCCTAATCCTAATACCAACTATCTCCCTAATTGAAA ACAAAATACTCAAATGGGCCT