DIY Micro Dispensing and Bio Printing

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Robotics, Pipettes Medias and more for Micro Dispensing and Bio Printing

Automatic liquid handling or microdispensing of small liquid amounts is useful in the production of biochips, Lab-on-a-Chip (LOC), biosensors and for tissue engineering. Crucial elements are: - Precise manipulation / XY(Z) robotics - Micro-dispensing and other formes liquid manipulation - Printing medias


First approaches during HackteriaLab 2011

Robotic Stage

HackStage V1

Small and precise mechanical manipulator made from recycled material and using open hard and software.

Basic principle:
- Two CD / DVD drives with stepper motor mounted cross wise
- Both drives are mounted fix, one is the object carrier the other is the manipulator
- Two stepper motor drivers (ex. pololu 4988) with micro stepping
- RepRap driver-software teacup on an arduino board
- G-Code may be generated with InkScape and G-Code plugin
- Controlled by ReplicatorG using G-Code


Printer in action video at: http://www.youtube.com/watch?v=E39qfKR-5s4

PrinterPrecision.jpg

Calibration Measurement. With a micro stepping motor driver a precision of less than 60 um can be achieved.

ReplicatorGScreen.jpg

Screenshot with ReplicatorG and WebCam image

PrintedSample2.jpg

"Printed" sample

MicroDropEPF.jpg

Comercial Dispensing Robot from MicroDrop at Ecole Polytechnique Federal Lausanne (EPFL)

Can be used for:
- Laser cutting masks or light materials using a DVD-laser-diode
Here an istruction on how to make a laser cutter: DIY Micro Laser Cutter
- Manipulate organisms or material
- Stage for microscopy
- Tissue engineering
(yeah yeah we print some fungi :-)
- Mini extruder
- SMD pick and place / stencil cutter

MicroManipulator.png

Some link to sources:

RepRap G-Code: http://reprap.org/wiki/G-code#T:_Select_Tool
Teacup Firmware: http://reprap.org/wiki/Teacup_Firmware
Pololu Motor Driver: http://www.pololu.com/catalog/product/1201
InkScape to G-Code: http://wiki.linuxcnc.org/cgi-bin/emcinfo.pl?InkscapeHowto
ReplicatorG Printing Program http://replicat.org/


Micro-Dispensing

Printing tip mad from a glas tube
FirstTip.jpg

First try printing tip made from glas tubing

DIYPiezoNozzle.jpg

First try DIY piezo nozzle (does not work properly)

Tip holding mechanism

Printing medias

First approaches during HackteriaLab 2011
FunghiOnPoly.jpg

Funghi growing on polyvinyl with LB-Medium

Finally urs got it done, super awesome

Related Links

DIY Laser Cutter: http://www.flickr.com/photos/imajilon/2210335514/in/photostream/
RepRap Laser Sintering: http://builders.reprap.org/2010/08/selective-laser-sintering-part-8.html
RepRap Tissue Engineering: http://forums.reprap.org/list.php?194
DIY Piezo Printhead http://reprap.org/wiki/Scratchbuilt_Piezo_Printhead
Comercial dispensing robots and piezo nozzles http://www.microdrop.de/
Growing Body Parts in the Lab Becomes Reality http://www.worldhealth.net/news/growing_body_parts_in_the_lab_becomes_re/
Controlling FIAlab Devices for Microfluidics http://88proof.com/synthetic_biology/blog/archives/407
Are Mushrooms The New Plastic http://www.ted.com/talks/eben_bayer_are_mushrooms_the_new_plastic.html
easy-to-use atomic force microscopes (AFM) and scanning tunneling microscopes (STM) http://www.nanosurf.com/
Hollow cantilever for liquid dispensing http://www.cytosurge.com/
General cell printing using an ink-jet http://www.sciencedaily.com/releases/2012/03/120316112648.htm