HLab14-Workshopology

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= Workshop Slots @ KKF =

Kaosologi
Isrol and friends

NanoDrop
see more info on DIY NanoDrop



Urs, Matt

Thoughts after session (by Matt):

Worked well, everyone completed the nanodrop and saw it splitting a spectrum. Could see a clear difference between pure colour filters (red/blue/green), and could see a difference between a pink solution and water. Need to consider the flatness of the LED spectrum, and the properties of the webcam, in turning the spectrum into an absolute measure of absorbance.

Most useful may be an A280 measurement (to measure amount of protein), but as this is below visible light, may be hard to detect. Perhaps try a range of webcams? We tried with 405 nm LEDs, and could see a peak around 400, but 280 nm was below our current detection.

Very promising though, requires a followup on properties of diffractive gratings, and perhaps a hunt for webcams/LEDs that can optimise and calibrate for an absolute measurement. For now thought, would detect presence of absorbant species, such as fluorophore dye, and probably would work to detect low concentrations of transition metals, which are very bright in the visible region, and also correspond to contamination in some instances (link to water contaminant testing?)

Book Binding
Tarlen

Learn how to make your own note book.

Beyond DIY Microscopy
5pm start.

Dipesh, Matt, Suparmin to demonstrate to students and public how to build a Micam 1.0 and Micam 2.0 (from Naaf Instruments).

Follow up session developing methods to improve microscope, such as adding a light condenser, building a phase contrast microscope, and a Differential Interference Contrast microscope.

This guy has already had a good attempt at a 'Nomarski-like' microscope: DIY D.I.C. He also has a good discussion of condensor filters here which explains what is going on. We could probably get away with just creating some circular filters and putting them in front of the LED, but building a condensor may well be worth it anyway.

We did end up taking some images using the lens from another webcam to form a makeshift condensor, and writing directly onto the back of the lens with coloured marker to try and make a back focal plane mask. The comparative results are shown below: Image of daphnia with no condensor or mask

Image of daphnia with mask in back focal plane (red centre, green periphery)

Balloonology
Togar, Urs, Yashas

biological immortality + alternative reproduction discussion + Humalga
the discussion will be from 7 pm till 9 pm

Join us on a discussion regarding the immortality/biological immortality + alternative reproduction discussion - we will present also research project Humalga  (by Spela & Robertina) dealing with this topic.

with Robertina, Spela, Gjino, Adeline

keywords: - immortality // biological immortality - aging - regeneration - regeneration medicine - alternative reproduction

useful links:

Nature's guide to immortality

Can a Jellyfish Unlock the Secret of Immortality? 

Humalga:

DIYbio Lab introduction 16:00-16:30
Get a short introduction to the Hlab14 Biolab or our Kitchen lab. We will share our best links, guides websites and a few advices to get started.

Make a diy-biolab

16.30-19 | Build a Bacterial Battery - Tweaking with bioelectronics
by[Gjino Sutic (UR Institute) & Martin Malthe Borch (Biologigaragen / IT-University of CPH

Learn the basics of biotweaking & bioelectronics by making Bacterial batteries, also known as Microbial Fuel cells..

Come and see how to hack the bacteria to generate power from organic waste, or how to use the same tech for water purification.. During the workshop we will play with different designs & you will see experimental bioelectronics that we are doing research on @UR Institute & Biologigaragen

Read more on "How to build a DIY Microbial Fuel Cells"

19 - 21h | Cockroches & Cookies
by Fajar Abadi, Timbil and friends

16 - 21h | DNA Barcoding
Adeline Gjino and Julito

We will discuss what barcoding means, molecular techniques involved, what it can be used for and limitations.

Can be used for: Protecting endangered species, Enforcing truthful labeling of food in the marketplace, Testing water quality, Regulating labeling of medicinal plants and herbal remedies, Preventing the international introduction of agricultural pest species.

Schedule:

4-6pm Intro to Barcoding, Species Concepts and Barcoding applications. DNA extraction or PCR!

6pm-7pm: Break/Dinner

7-9pm: Run Gel and More Discussions

General guidelines on sample collection for DNA analysis:

PCR reagents used: PCR steps:

Google funding barcoding:

more ideas about DNA Barcoding

15 - 19h | Full Panorama Photography and Lens Geeking
Yung-chieh 林永杰 Photographer and animator. Particularly interested in micro-photography and panorama, with self-invented and hacked photography equipment. He will introduce his DIY Photo equipment and go for walks around KKF with the participants to explore the uses of his panorama photography.

Together with Budi Laksono, from X-code and Mary Tsang, DIY-sect, we will further discuss various experimental approaches to photography. Bring your own camera for more lens geeking and photography discussions.

15 - 19h | Bottle Hacking / Bring your own Beer
Ivan Bestari, Otakatik Creative Workshop, will introduce basic skills of recyling glass bottles for making new things.

11.30 - 23h | FULL-DAY SEXOLOGY WORKSHOP
Pechblenda, Fred Kuang-Yi_Ku, Timbil, Cindy Lin This is a half-day workshop speaking about issues related with body, technology and desire. A few of these open workshops speak about DIY mechanisms related with pleasure-giving and receiving while still being connected to the research nodes artists/scientists/hackers/makers have been exposed to during Hlab14. Others are more concerned with sexual intimacy, genitalia dryness and hygiene. While such issues are often spoken less about, these workshops hope to communicate with participants about issues surrounding re-embodying DIY culture, the gendered nature of DIWO and other gender and sexual-related issues in hackerspaces.

 DILDOMANCY 

Make your microorganism dildo for macropleasure!

For more details, please visit DILDOMANCY ! DIY Personal LUBRIKASI (Lubricant) This workshop aims to educate participants about making personal lubricants not only to ensure that lesser friction occurs during sexual intercourse but also for application on sex toys to guarantee ease in penetration or other forms of usage deemed favorable for user. More importantly, the usage of DIY water-based lube makes it more difficult for condoms and sex toys to break down and other user-unfriendly reasons oil lubricant users may encounter.

Viscosity of DIY lubricant has already been standardized by workshop givers. However, this can be altered according to personal preference. Coloured and flavoured essences for experimentation will be introduced to create a variety of lubricants!

IN-BETWEEN AROMATHERAPY: Genitalia Spa Hacking In this workshop, we will combine knowledge from traditional Javanese methods popularly known for cleansing and perfuming genitalia (vagina/penis/intersex) and traditional/contemporary knowledge of flavouring natural concoctions a.k.a. gastronomic way of flavouring to temporally alter smells. This workshop does not aim to advocate genitalia steaming as necessarily beneficial for fertility, blood circulation and other health benefits promised by vaginal spa treatments in Yogyakarta but aims to understand our genitalia better through unfamiliar environments.

Daun Sirih (Sirih leaves) will be used together with various flavoured essences in a for genitalia steaming. Other fragrant plants and herbs will be used according to genitalia steaming recipes offered by contemporary Javanese spa salons and bars. All of these recipes are open for alteration and sharing. All men/women/transgender/genderfluid/genderfuck participants are welcomed.

= Workshop Slots @ BPR = Various workshops from 19 - 23. April, primarily focusing on working with children in the neighborhood. Age 5-16

FLOFA BOT // Flora Fauna Bot by Helmi Hardian & Debrina Tedja
In this class, the kids will observe around about organism, and then give presentation. After that we encourage them to be imaginative and create a story, either drawing or writing it which explains this question: ‘If you can create any kind of creatures (animals, plants, alien, ghost, mutant, or anything), what it will be and describe them?’ Then they can start to make this creature with Flofa bots, which is made of brush and motor, so it can move, also decorate it from scratch and waste. In the end, we asked them to have short presentation about their creature and make a racing game.

Format : 1-2 hours, suitable for 8-10 years old, and the maximum number of participants is 10.

Support Material : Crayons, Colored Pencils, Colored Markers, Colored Papers, Ice Cream Sticks, Buttons, Ribbons, Movable Eyes, Toothpicks, Glue etc

++ : White board + Black Board Marker (the fattest one)

BIOSYNTHS – BUILDING ELECTRONIC MUSICAL INSTRUMENTS WITH NATURE

 * Responsible Mentor: Pia van Gelder

Synthesizers are generally played using knobs, switches and keyboards. What happens when natural events and phenomena are used as control synthesizers? Can we design instruments that are played by nature? In this workshop we will build our own hand-made synthesizers and we will examine how these synthesizers can be controlled by natural and biological systems so that we can listen to the music nature makes.


 * material support needed?
 * assistant other than translator?
 * format, fix time? approx how long? / ongoing? approx how long?
 * suitable age n ideal number of kids can take part ?

Mechanical Sculpture and 3D Printing By Michael Candy
A short tutorial in basic mechanical principals will be applied in a practical manner as participants are encouraged to make small kinetic contraptions and generators powered by rubber bands. Complex components in the devices (such as pulleys or gears) will be 3D printed in situe and some participants will have the opportunity to design their own unique parts for their installation.

Format: 3 - 4h depending on kids' motivtaion. Preferably indoor classroom setup (or something similar) to begin with. And later heading outdoors to test and play with the sculptures created. Suitable for age 7 and up, some aspects of the workshop might be slightly complex for under 7’s, best around 15 students (to be put into groups of 3)

Support Material: I will organize and supply materials

I will be bringing a small 3D printer for this workshop (so we need electricity!)

I do not necessarily need a projector! This was only to display 3D designs on my laptop, this can be done fine with just the laptop screen. – white board or blackboard or maybe even some paper could be good to demonstrate some idea’s / principals about mechanics.

Carnival game by Dipeshwor Shrestha & Karkhana
Kids will learn about Design cycle called the TMPI (Think, Make, Play, Improve). Their task is to make a carnival game in 40 minutes. They will first generate ideas, discuss it with their peers and then create a cardboard prototype. Finally, they will present their games to the whole class and everyone will play each others games. Additionally, the class will teach the students about energy transformations that are being used in the games they make.

Format: 3h, 1 lead teacher with approx. 2 helpers for 30 kids

Support Material: 1. projector (we can think of other ways to present if a projector is not available), 2. cardboard, 3. rubber bands, 4. paper cutter, 5. hot glue gun + glue, 6. duct tape, 7. table tennis balls, 8. scissors, 9. Lego blocks (enough for 30 kids), 10. color paper ~

Carnival game : (9:00 am to 12:30 pm, April 20, 2014)  Karkhana in collaboration with Waft Lab and undergraduate students from the Indonesian universities conducted a workshop at the BPR for kids. There were 20 kids, roughly 6 to 12 years old. The Karkhana team form Nepal tried to transfer their knowledge and teaching experience to the Indonesian team so that they could conduct these kinds of workshops for the local kids. We had a wonderful Indonesian team from Waft and volunteers from Indonesian universities that ran the class. The slides were converted to Basha Indonesia from English beforehand. We first started by playing the 'WAAA' game. It was a fun experience and we got to know the children better even though we did not speak any Basha and the kids did not speak any English. The first activity the kids did was to build the tallest tower and then the widest tower using lego blocks. After the activity, we explained the kids the TMPI (Think, Make, Play, Improve) cycle or the BBMK (Bayankan, Buatlah, Mainkan, Kembangkan) cycle in Indonesian. After that the kids made games using tools like paper cutter, hot gun, scissors, markers and materials like cardboard, baloons, table tennis balls, paper along with locally available materials like bamboo. Here's the list of the games they made. 1. Bow and Arrow The kids made the bow and arrow using bamboo. We were impressed by the skills the kids had in using the tools. The player had to hit the eyes of the target. There were two balloons behind the eyes that would burst if the player hit the target. The bow and arrow were well made. And the kids made mustache and beard from duct tapes to pose as the village elders/experienced hunters. 2. Fish in the pond The kids made a fishing rod from bamboo and string and the fish using cardboard. They placed small magnets inside the fish. The players had to catch as many fish as they could using the fishing rod which also had a magnet as a hook. 3. Board game The kids made a carrom board which two player could play. They used markers to color the chips and used bamboo to reinforce the structure of the board. 4. Bowling The kids used cardboard to make bowling pins. The player had to throw a table tennis ball and strike the pins to score points. 5. Footall The kids made a two player game similar to football but instead of their feet, the players used a bat to score goals against each other. Another nice thing about this game was that the winners got prizes. (I won a big balloon. :D )

The kids had a great time. They made these amazing games in a few hours and used the TMPI (BBMK) cycle. Some participants form Hackteria played their games and gave feedback to the kids on how to improve their games.

We ended the session with a general discussion in which the teachers/facilitators (from Nepal and Indonesia) talked about two/three things that were good (what went well) and two/three thing that could be improved (even better if).

=Further Ideas=

BioCyberKidzz, Maribor

 * Responsible: tba from participant call

A body hack is a usable technique to improve a person's life or physical capabilities. Hacking the human body has become quite commonplace. Many people have body modifications such as tattoos, piercings or even advanced medical prosthetics. Simple and playful body modifications that allow enhancing our human performance can be made with just few and cheap materials, such as cardboard, magnets and LEDs. The Bio-Acoustics Ear amplifier can augment a directional perception of sound. With a Disco-on-a-Fingertip we can modify our fingers into torches, disco lights or fluorescence detectors and we can augment our touch perception and add a new sense of magnetism by building body extension’s such as the Magnetic Finger. Or finally, by mixing algae with agar or other jelly we can make a Photosynthetic Skin. These body modifications will be observed and recorded in The Lab Book. The children will be introduced to a playful laboratory setting where the lab becomes less of an intimidating space but more an environment to spark their curiosity.' The BioCyberKidzz workshop has been developed as a result of HackteriaLab 2011 by collective brainstorms of many individuals and communities, that are devoting their rich experiences in creating alternative crossovers between art and science. It has been invited to festivals such as Ars Electronica 2011 and others.

See BioCyberKidzz, Maribor for material details and images.

= Workshopology Discussion Round (tba) =

More info soon

=Workshopology Commons=

Please put down general ideas and topics for doing Workshops.

Cheese Making
See more on the hackteria website

by dusjagr and friends

General Ferments
by lifepatch and friends

Soap Making
by tamara

DIY microscopy
I've put most of the notes on the microscopy workshop above, in the workshop description:

Workshopology

by UGM students

OpenNanoDrop
by Urs Gaudenz

DIY media for growing environmental samples
(Experimental Shroom Lab, Plant tissue culturing/cloning, Antibiotic/Antifungal screening of plants etc.)

by Gjino, Malte, Adeline, Sachiko, Pechblenda et al.

http://ag.arizona.edu/classes/plp427L/lab2.html http://www.life-worldwide.org/fungal-diseases/fungal-culture/ http://www.fungifun.org/English/Pftek https://dl.dropboxusercontent.com/u/53879632/DIY%20Patato%20dextrose%20agar.pdf

Mobile Lab Soundsystem
by Matt and James (begin build at 3pm 18/4/2014)

Building a backpack soundsystem that can be carried easily by one person, but play music for > 12 hours to provide atmosphere for field research.

Plan is to reinforce backpack with plywood, use stereo amplifier and cross filter to split to low and high range, and run all from 12V with no conversion to maximise energy efficiency and duration vs weight.