User:Sem2490

Sandeep Eli Mathew

2nd year Foundation Studies Srishti School of Art Design and Technology

sem2490 at gmail.com

Bacteria with a memmory


When the bacteria senses red light, something turns on. At any later time, when the bacteria senses green light, it glows red. The bacteria has to remember that the red light was the first colour it sensed. It should work vice versa. I still have to figure out how to make a part remember something. Maybe, it can produce a protein, amd the proteins presence triggers another part. The part that senses red light.

Bacterial paint
A source of light will produce red, yellow and blue light. When the light is shone on the bacteria, it will produce pigments of the same color as the light. Most pigments are fat soluble, well at least this red pigment.

Red Lemon Flavoured Bacteria
The parts that are a red pigment producing operon and a Limonene synthase gene.( kit 2 20E) They both need farnesyl diphosphate which is normally made be e. coli (incidentally, farnesyl diphosate is also required for the synthesis of Geosmin.) Turns out the pigment producing parts are still in the planning stage. For now Lysis shall happen:Part:BBa_I742109(kit 2 	14D)

22nd July
We have been trying to kill E coli cells for the past 3 weeks. We could just soak them in alcohol or something, but we aren't that lame and boring, are we? We want the bacteria to lyse, to kill itself from the inside, oh the profoundness. Well then, to do that we had to get a lysis gene in there. The thing is, with the lysis gene needs to be turned on by this thing called a promoter. There are two kinds of promoters, ones that make the gene they're attached to go all the time-but whats the use of that? There has to be a whole big show and what not, we dont want the actors to die before the big death scene now do we? (uh, yes, ignore). Then, you have ones that turn on when you want them to, in our case, when you add a mystery ensyme. So, that gets things more complicated. For details look at Week Four. After three weeks for protocol following and waiting, we got nothing. Apparently one of the enzymes involved in the process isn't working too well. Oh well.