SynthBioWorkshop2010

&lt;strong&gt;Synthetic Biology for Artists and Designers 2&lt;/strong&gt;

&lt;strong&gt;10th May 2010 &lt;/strong&gt; • Biology - individual understanding and definition. • Brief History of Biology - Scientists/Microbiologists (Video) ? Anton Von Leeuwenhoek ? Gregor Mendel ? Robert Hooke ? Louis Pasteur ? John Baptiste Van Helmont ? Joseph Jackson Lister ? Theodore Schwann ? Rosalyn Franklin ? Watson &amp; Crick • Evolution – Brief understanding and controversies • DNA, Protein – Introduction • Bacteria E.Coli • Readings – PDF ? Scientific America – Engineering Life ? Synthesizing DNA of microorganisms for various purposes ? Sequencing of DNA ? Cost factors involved ? Comparison to Moore’s Law ? Human Genome • Video – (BBC)-The Cell-The Hidden Kingdom Homework - Reading (PDF) 1. Life: What a concept!

&lt;strong&gt;11th May 2010&lt;/strong&gt;

• Recap of yesterday’s learning • Video lecture by David Sadava ? Expression of genes ? Phenotypes and Alleles ? The Nucleus ? Totipotent Cells ? Cloning ? Genes, chromosomes ? Human genetic information ? Cell processing • Introduction to Synthetic Biology by Andrew Hessels • Video – Darwin’s theory on evolution Homework 1.      2.

&lt;strong&gt;12th May 2010&lt;/strong&gt;

• Video Lecture by David Sadava ? Epidemiology ? Lung Cancer gene ? Mutations ? Requirements of a DNA ? Responsibilities of a Protein ? Variety in DNA ? Somatic and Sex Cells ? Virus ? DNA &amp; its properties ? Structure of a DNA ? Replication of DNA • Video of Watson and his story of the discovery of the DNA model at TED.com • Readings – Applying engineering to biology • Reading – Adventures in Synthetic Biology Homework 1. Reading 2. Online Research

&lt;strong&gt;13th May 2010&lt;/strong&gt;

1. Video Lecture by David Sadava ? Spider web theory of protein ? Protein production ? Structure of protein ? Synthesis of Protein ? Human protein synthesis ? Types of proteins ? Expression of Proteins ? Gene – Protein relationship ? Metabolism ? Thermodynamics ? Mechanism, Cell Theory and Evolution ? Ricin and its discovery ? RNA and its role ? Transcription and Translation ? Nirenberg’s Experiment ? Mutations and changes in base pairs • Video tutorial on DNA replication • Video tutorial on Protein synthesis Homework 1. Exercise – Make a piece of art form using any LIVING form that should have the capability to grow over a period of two months.

&lt;strong&gt;14th May 2010&lt;/strong&gt;

1. Video Lecture by David Sadava Genomes – further details ?      Mutations &amp; Mutagens ?      Cancers caused by mutation of genes by radiation ?      Genetic Damage ?      Leukaemia ?      Scientist Renato del Bucco’s work ?      Hierarchical sequencing ?      Shotgun sequencing – Craig Ventor ?      Bioinformatics ?      Information from a genetic sequence ?      a. Coding sequence b. Protein amino acid sequence c. Gene control sequences Sequential Genomics ?      First genome to be sequenced ?      Hypothetical suggestion of 24000 genes in a human being ?      Synthetic Biology - making artificial genes to bring about a desired result ?      Scientist Werner Arber ?      Restriction Endonuclease ?      How a bacteria prevents Viral infections ?      DNA Mapping ?      Recombinant DNA ?      Stanley Cohen &amp; Herbert Boyer’s Experiment ?      Goals for making rDNA ?      Transgenic animals and plants ?      Naked and Vector DNA ?

&lt;strong&gt;17th May 2010-05-17&lt;/strong&gt;

1. Video Lecture by David Sadava ? Isolation of genes ? PKU and dystrophy ? Methods of analyzing DNA ? Reverse Genetics ? Dystrophine Gene and Utrophine ? Gene Therapy ? Nucleic Acid Hybridization ? Genome Library ? DNA Microarray ? Metastasis 2. Exercise - Think of your own creature. Design it. Explain detailed mechanisms using diagrams. This organism could be hypothetical. Name it. 3. Make a scientific paper on your creature 4. Demonstration of Gabriel’s current prototype of visually representing genetic sequenced data of a human mitochondria. Homework – develop and complete creature.

&lt;strong&gt;18th May 2010&lt;/strong&gt;

1. Video Documentary – BBC – The Cell – Chemistry of Life 2. Video Documentary – BBC – The Cell – The Spark of Life 3. Discussion of Creatures, peer feedback and progress

&lt;strong&gt;19th May 2010&lt;/strong&gt;

&lt;object width=&quot;400&quot; height=&quot;300&quot;&gt;&lt;param name=&quot;allowfullscreen&quot; value=&quot;true&quot; /&gt;&lt;param name=&quot;allowscriptaccess&quot; value=&quot;always&quot; /&gt;&lt;param name=&quot;movie&quot; value=&quot;http://vimeo.com/moogaloop.swf?clip_id=12045337&amp;server=vimeo.com&amp;show_title=1&amp;show_byline=1&amp;show_portrait=0&amp;color=&amp;fullscreen=1&quot; /&gt;&lt;embed src=&quot;http://vimeo.com/moogaloop.swf?clip_id=12045337&amp;server=vimeo.com&amp;show_title=1&amp;show_byline=1&amp;show_portrait=0&amp;color=&amp;fullscreen=1&quot; type=&quot;application/x-shockwave-flash&quot; allowfullscreen=&quot;true&quot; allowscriptaccess=&quot;always&quot; width=&quot;400&quot; height=&quot;300&quot;&gt;&lt;/embed&gt;&lt;/object&gt;&lt;a href=&quot;http://vimeo.com/12045337&quot;&gt;Center for Genomic Gastronomy @ Srishti, Bangalore, India&lt;/a&gt; from &lt;a href=&quot;http://vimeo.com/user3901760&quot;&gt;genomic gastronomy&lt;/a&gt; on &lt;a href=&quot;http://vimeo.com&quot;&gt;Vimeo&lt;/a&gt;.

1. Presentation by Zac on The center for Genomic Gastronomy ? What is Biotechnolgy ? Perspective of food by all living things ? Geohackers, Biohackers, Geoengineers, Planetcrafters ? Agriculture Biodiversity ? Agrinformatics ? Metadata and food Genomes ? Food System Adaptation ? Agriculture Terrorism ? First world eating issues ? Cuisinformatics 2. Extraction of DNA

&lt;strong&gt;20th May 2010&lt;/strong&gt;

1. Presentation of Abstracts of New Creatures ? The Booster Cell ? Supercule ? Thermo Cell 2. Exercise – Create another new creature which does not necessarily have to be a       microorganism or even possible to exist. Completely hypothetical, imaginary of fantastical is       great!

&lt;strong&gt;21st May 2010&lt;/strong&gt;

Minutes of the Class 1. Discussion of ideation on creation of machines.

&lt;strong&gt;22nd May 2010&lt;/strong&gt;

Minutes of the Class 1. Video Documentary by Craig Ventor ? Scientific Breakthrough – First fully Synthetic Cell to be cultutred. ? Genome copied from Mycoplasma Genatalium ? Genome synthetically prepared in the lab ? Introduced into yeast cells to complete transformation ? Introduced into host bacterial cell which transforms to new organism ? Watermarked with 46 authors, web address and mail ID           ? Significance – vaccine production and Oil Spill reduction 2. Artist &amp; other Research ? Stellarc ? Symbiotica ? Orlan

&lt;strong&gt;25th May 2010&lt;/strong&gt;

1. Visit to NCBS ? Tour of the Campus ? Meeting with PhD student Mehra and Navneet ? Introduction to the lab space ? Discussion over Science Institutes and their pros and cons ? Tour of other labs with various workflow instruments

&lt;strong&gt;26th May 2010&lt;/strong&gt;

Minutes of the Class 1. Video Tutorial by David Sadava ? Bioremediation – using organism to improve the environment ? Fluorescence in nature and different organisms ? TNT used as example ? Degraded by Psuedomonas Putida ? Integrated Pest Management ? Bacteria in decomposition ? Bacteria in Waste Water Treatment ? Extremophiles ? PCR and its significance 2. List of materials required to build the art form made from a living organism 3. Artist &amp; other Research ? Institution of Applied Autonomy ? Center for Post Natural History ? George Gessert

&lt;strong&gt;27th May 2010&lt;/strong&gt;

Minutes of the Class 1. Video Documentary by Kary Mullis on the making of the PCR 2. Artist &amp; other Research ? Adam Zaretsky ? Eduardo Kac ? Patricia Piccinni 3. Field work on material for art form construction.

&lt;strong&gt; 1st June &lt;/strong&gt;

Yeast Growing http://www.disknet.com/indiana_biolab/b021.htm